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In Vivo Alkaline Comet Assay and Enzyme-modified Alkaline Comet Assay for Measuring DNA Strand Breaks and Oxidative DNA Damage in Rat Liver
 

In Vivo Alkaline Comet Assay and Enzyme-modified Alkaline Comet Assay for Measuring DNA Strand Breaks and Oxidative DNA Damage in Rat Liver

Article DOI: 10.3791/53833-v • 10:38 min • May 4th, 2016
May 4th, 2016 •
1, 1, 1, 2, 1
1Division of Genetic and Molecular Toxicology, US FDA/National Center for Toxicological Research, 2Toxicologic Pathology Associates, US FDA/National Center for Toxicological Research

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The alkaline comet assay measures DNA strand breaks in eukaryotic cells. By adding an Endonuclease III or human 8-oxoguanine-DNA-N-glycosylase digestion step, the assay can efficiently detect oxidative DNA damage. We describe methods for using these assays to detect DNA damage in rat liver.

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In Vivo Alkaline Comet Assay Enzyme-modified Alkaline Comet Assay DNA Strand Breaks Oxidative DNA Damage Rat Liver Safety Analysis FDA Regulated Products Regulatory Safety Evaluation Molecular Epidemiology Basic Cancer Research Sensitive Versatile Inexpensive Single-cell Level Animal Tissue Single Cell Suspensions Visual Demonstration Carbon Dioxide Gas Euthanasia Palpate Heartbeat Pinch Toes Disinfect Skin Surgical Scissors Abdominal Cavity Intact Liver
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