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Developmental Biology

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JoVE Journal Developmental Biology
Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells (ASCs)
 

Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells (ASCs)

Article DOI: 10.3791/53890-v • 08:52 min • August 11th, 2016
August 11th, 2016 •
1, 1
1Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes, Biointerfaces Institute, University of Michigan

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We present the techniques required to isolate the stromal vascular fraction (SVF) from mouse inguinal (subcutaneous) and perigonadal (visceral) adipose tissue depots to assess their gene expression and collagenolytic activity. This method includes the enrichment of Sca1high adipose-derived stem cells (ASCs) using immunomagnetic cell separation.

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Immunomagnetic Separation Fat Depot-specific Sca1high Adipose-derived Stem Cells ASCs Cell Sorting Adipose-derived Stem Cells Fat Depots Collagenase-mediated Tissue Digestion Subcutaneous Fat Pads Foam Dissecting Board 70% Ethanol Small Incision Lower Abdomen Peritoneum Thorax Lateral Cuts In The Skin Inguinal Fat Pad
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