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JoVE Journal Developmental Biology
Isolation, Culture, and Adipogenic Induction of Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue
 

Isolation, Culture, and Adipogenic Induction of Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue

Article DOI: 10.3791/60691-v • 08:31 min • March 2nd, 2020
March 2nd, 2020 •
*1, *2, 3, 3, 4, 5, 4, 5, 1
1Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, 2Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 3Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 4Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 5Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine, Huadong Hospital Affiliated to Fudan University
* These authors contributed equally

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résumé
Automatic Translation

We present a protocol for the isolation, culture, and adipogenic induction of neural crest derived adipose-derived stem cells (NCADSCs) from the periaortic adipose tissue of Wnt-1 Cre+/-;Rosa26RFP/+ mice. The NCADSCs can be an easily accessible source of ADSCs for modeling adipogenesis or lipogenesis in vitro.

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Isolation Culture Adipogenic Induction Neural Crest Adipose-derived Stem Cells Periaortic Adipose Tissue Methodology PVAT Adipogenesis Drugs Against Obesity Cardiovascular Diseases Fluorescence NCADSCs Biotin Labeling FACS Magnetic Activated Cell Sorting Ectoderm Lipogenesis Young Mice Cautery Induced Adipogenesis 3T3 Cells PAAT Dissection Sterilizing Mouse Carcass Scissors Abdomen Ventral Midline Pelvis To Neck Diaphragm Ribs Heart And Lungs Extraction
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