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Immunology and Infection

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JoVE Journal Immunology and Infection
The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids
 

The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids

Article DOI: 10.3791/54033-v • 09:09 min • May 18th, 2016
May 18th, 2016 •
1, 2, 1, 2,3,4, 1
1Department of Biomedical Sciences and Pathobiology, Virginia Maryland College of Veterinary Medicine, Virginia Tech, 2Department of Biomedical Engineering, Cornell University, 3School of Electrical and Computer Engineering, Cornell University, 4Department of Biomedical Engineering, Duke University

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résumé
Automatic Translation

Here, a protocol to harvest, maintain, and treat mouse small intestinal organoids with pathogen associated molecular patterns (PAMPs) and Listeria monocytogenes is described, as well as emphasis on gene expression and proper normalization techniques for protein.

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Keywords: Mouse Intestinal Organoids Ex Vivo Culture Pattern Recognition Receptor Stimulation Innate Immunity Mucosal Immunology Host-pathogen Interactions Intestinal Epithelial Cells ELISA Intestinal Crypts Normalization Cell Number PBS EDTA DMEM/F12 Cell Fractionation Crypt Visualization
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