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Developmental Biology

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JoVE Journal Developmental Biology
Generation af induceret-pluripotente stamceller Brug fibroblastlignende synoviocytter Isoleret fra leddene på leddegigt patienter
 
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Generation af induceret-pluripotente stamceller Brug fibroblastlignende synoviocytter Isoleret fra leddene på leddegigt patienter

Article DOI: 10.3791/54072-v • 09:31 min • October 16th, 2016
October 16th, 2016 •
1,3, 1, 1, 2,3
1CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Division of Rheumatology, Seoul St. Mary's Hospital, Republic of Korea, 2Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary's Hospital, Institute of Medical Science, Republic of Korea, 3College of Medicine, The Catholic University of Korea, Republic of Korea

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Her beskriver vi en protokol til generering humane induceret pluripotente stamceller fra patient-afledt fibroblastlignende synoviocytter, ved anvendelse af en lentiviral system uden fødeceller.

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Developmental Biology induceret pluripotente stamcelle leddegigt fibroblast-lignende synoviocyt lentivirus stamceller biologi omprogrammering feeder-fri
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