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Biochemistry

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JoVE Journal Biochemistry
High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
 

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

Article DOI: 10.3791/54286-v • 06:51 min • September 20th, 2016
September 20th, 2016 •
*1, *1, 2
1Department for Plant and Environmental Sciences, University of Copenhagen, 2School of Agriculture, Food and Rural Development, Newcastle University
* These authors contributed equally

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résumé
Automatic Translation

A high-throughput assay for enzyme screening is described. This multiplexed ready-to-use assay kit comprises of pre-chosen Chromogenic Polymer Hydrogel (CPH) substrates and complex Insoluble Chromogenic Biomass (ICB) substrates. Target enzymes are polysaccharide degrading endo-enzymes and proteases.

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Keywords: High-throughput Screening Carbohydrate-degrading Enzymes Novel Insoluble Chromogenic Substrate Assay Kits Enzyme Discovery Biomass Degradation Chromogenic Substrates Endo-cellulase ICB-wheat Straw Absorbance Plate Reader
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