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Comparison of Tobacco Host Cell Protein Removal Methods by Blanching Intact Plants or by Heat Treatment of Extracts
 

Comparison of Tobacco Host Cell Protein Removal Methods by Blanching Intact Plants or by Heat Treatment of Extracts

Article DOI: 10.3791/54343-v • 11:06 min • August 8th, 2016
August 8th, 2016 •
1,2, 3, 1,2
1Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V., 2Institute for Molecular Biotechnology, RWTH Aachen University, 3Department of Biomolecular Separation Engineering, Karlsruhe Institute of Technology (KIT)

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résumé
Automatic Translation

Three heat precipitation methods are presented that effectively remove more than 90% of host cell proteins (HCPs) from tobacco extracts prior to any other purification step. The plant HCPs irreversibly aggregate at temperatures above 60 °C.

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Keywords: Tobacco Host Cell Proteins Heat Treatment Blanching Protein Removal Purification Recombinant Biopharmaceuticals Proteases Extraction Water Bath Temperature Control Incubation Time
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