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Neuroscience

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JoVE Journal Neuroscience
Automated Analysis of C. elegans Swim Behavior Using CeleST Software
 

Automated Analysis of C. elegans Swim Behavior Using CeleST Software

Article DOI: 10.3791/54359-v • 08:47 min • December 7th, 2016
December 7th, 2016 •
1, 2, 1, 2, 1
1Department of Molecular Biology and Biochemistry, Rutgers University, 2Computing Research and Education Building (CoRE), Rutgers University

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résumé
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An efficient and simple methodology for computer-based analysis of nematode swimming behavior in liquid is described. The method requires little to no investment for C. elegans laboratories. The hardware used is standard, and the computer software for the behavioral analysis (CeleST) is an open source one.

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Automated Analysis C. Elegans Swim Behavior CeleST Software Computer Vision Software C. Elegans Swim Test Genetic Mutations Pharmacological Interventions Locomotive Capacities Swim Posture Analysis Eight Parameters Of Swim Behavior Automated Data Evaluation Stereo Microscope Digital CCD Camera Digital Video Recording Software Computer Vision Software Swim Drop Area Microscope Slide Image Quality Working Distance Magnification Base Mirror Dark Field Illumination M9 Buffer
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