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Assays to Detect UV-reflecting Structures and Determine their Importance in Mate Preference using the Sailfin Molly Poecilia latipinna
 

Assays to Detect UV-reflecting Structures and Determine their Importance in Mate Preference using the Sailfin Molly Poecilia latipinna

Article DOI: 10.3791/54453-v • 06:41 min • September 14th, 2016
September 14th, 2016 •
1, 2
1Department of Zoology, Ohio Wesleyan University, 2Department of Ecology, Evolution, & Behavior, University of Minnesota

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This protocol outlines the use of spectrophotometry to detect ultraviolet-reflecting structures on organisms (in this example, the sailfin molly Poecilia latipinna) and describes dichotomous choice tests for fish that allow inferences to be made on the role of ultraviolet cues during mate selection.

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Assays Detect UV-reflecting Structures Mate Preference Sailfin Molly (Poecilia Latipinna) UV Reflectance Mating Behavior UV Cues Spectrophotometer Calibration Light Source Calibration Anesthetize Fish Ethyl 3-aminobenzoate Methane Sulfonic Acid Salt (MS222) Sodium Bicarbonate Black Non-reflective Background Dim Room Lights Reflectance Data Recording Analysis Software Integration Time Setting Average Number Of Scans Setting Pixel Smoothing Value
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