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Creation and Transplantation of an Adipose-derived Stem Cell (ASC) Sheet in a Diabetic Wound-healing Model
 

Creation and Transplantation of an Adipose-derived Stem Cell (ASC) Sheet in a Diabetic Wound-healing Model

Article DOI: 10.3791/54539-v • 08:06 min • August 4th, 2017
August 4th, 2017 •
1, 2, 2, 2, 2, 3, 1, 1, 2, 2, 2, 1
1Diabetic Center, Tokyo Women's Medical University School of Medicine, 2The Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 3The Department of Anatomy and Developmental Biology, Tokyo Women's Medical University School of Medicine

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résumé
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Adipose-derived stem cells (ASCs) are easily isolated and harvested from the fat of normal rats. ASC sheets can be created using cell-sheet engineering and can be transplanted into Zucker diabetic fatty rats exhibiting full-thickness skin defects with exposed bone and then covered with a bilayer of artificial skin.

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Adipose-derived Stem Cell ASC Sheet Diabetic Wound Healing Artificial Skin Chronic Wound Large Full-thickness Skin Defect Cell Sheet Technology Solid Sheets Transplant Lewis Rats Surgical Site Sterile Cotton-tipped Applicators Gauzes Scalpel Needle Holder Operating Scissors Forceps 5-0 Nylon Suture PBS (phosphate-buffered Saline) Anesthetizing The Rat Supine Position Sterile Drape Shave Operative Area Scrub Exposed Skin Skin Incision Lateral Lower Abdomen External Oblique Muscle Epididymal Adipose Tissue
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