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Developmental Biology

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JoVE Journal Developmental Biology
Imaging of Cell Shape Alteration and Cell Movement in Drosophila Gastrulation Using DE-cadherin Reporter Transgenic Flies
 

Imaging of Cell Shape Alteration and Cell Movement in Drosophila Gastrulation Using DE-cadherin Reporter Transgenic Flies

Article DOI: 10.3791/54764-v • 04:42 min • December 29th, 2016
December 29th, 2016 •
1, 2, 3, 2, 3,4,5
1Biotechnology and Genetic Engineering Department, Jahangirnagar University, 2JT Biohistory Research Hall, 3Division of Cell Regeneration and Transplantation, School of Medicine, Nihon University, 4Graduate School of Life and Medical Sciences, Doshisha University, 5Institute of Genetics and Animal Breeding of the Polish Academy of Sciences

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Herein we describe a procedure to capture live images of Drosophila gastrulation. This has enabled us to better understand the apical constriction involved in early development and further analyze mechanisms governing cellular movements during tissue structure modification.

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Keywords: Cell Shape Cell Movement Drosophila Gastrulation DE-cadherin Reporter Transgenic Flies Cellular Rearrangement Apical Constriction Embryonic Development Imaging Microscopy Sample Preparation Embryo Collection Dechorionation Orientation Mounting
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