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Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy
 

Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy

Article DOI: 10.3791/54975-v 05:44 min March 6th, 2017
March 6th, 2017

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résumé

Here we present a protocol to measure the force of interactions between a well-defined inorganic surface and either peptides or amino acids by single-molecule force spectroscopy measurements using an atomic force microscope (AFM). The information obtained from the measurement is important to better understand the peptide-inorganic material interphase.

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Keywords: Single-molecule Force Spectroscopy Amino Acids Peptides Inorganic Materials Surface Chemistry Composites Chordates AFM Cantilevers Silane Compounds Fmoc-PEG-NHS Piperidine N-Methyl-2-pyrrolidone Amino Acid Coupling
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