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Immunology and Infection

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JoVE Journal Immunology and Infection
Dextran effektiviserar Lentiviral transduktion murina och mänskliga primära NK-celler
 
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Dextran effektiviserar Lentiviral transduktion murina och mänskliga primära NK-celler

Article DOI: 10.3791/55063-v • 08:42 min • January 15th, 2018
January 15th, 2018 •
1, 2, 3, 4, 5, 6, 6, 1,5,6,7
1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute, The Blood Center of Wisconsin, 2Vector Core Lab, Blood Research Institute, The Blood Center of Wisconsin, 3Laboratory of Lymphocyte Biology, Blood Research Institute, The Blood Center of Wisconsin, 4Laboratory of Stem Cell Transcriptional Regulation, Blood Research Institute, The Blood Center of Wisconsin, 5Department of Microbiology and Immunology, The Medical College of Wisconsin, 6Department of Pediatrics, The Medical College of Wisconsin, 7Department of Medicine, The Medical College of Wisconsin

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Målet med denna studie var att formulera teknik som möjliggör framgångsrik gen transduktion i primära natural killer (NK) celler. Av dextran-medierad lentiviral transduktion av mänskliga eller mus primära NK celler resultat i högre gen uttryck effektivitet. Denna metod av genen transduktion kommer att avsevärt förbättra NK cell genetisk manipulation.

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Immunologi fråga 131 transduktion primära NK-celler dextran lentivirus genetiskt modifierade immunterapi
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