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Biochemistry

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JoVE Journal Biochemistry
A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis
 

A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis

Article DOI: 10.3791/55077-v • 05:43 min • January 24th, 2017
January 24th, 2017 •
*1, *2, 3, 1, 3,4,5
1Graduate School of Frontier Biosciences, Osaka University, 2Department of Biotechnology and Genetic Engineering, Jahangirnagar University, 3Division of Cell Regeneration and Transplantation, School of Medicine, Nihon University, 4Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, 5Graduate School of Life and Medical Sciences, Doshisha University
* These authors contributed equally

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résumé
Automatic Translation

We have established a method for the purification of coregulatory interaction proteins using the LC-MS/MS system.

Tags

Protein Preparation Method High-throughput Identification Proteins Interacting Nuclear Cofactor LC-MS/MS Analysis Transcriptional Mechanisms Gene Regulation Advantage Technique Transcriptional Regulatory Mechanisms HEK 293 Cells Flag-tagged ARIP4 Transvection Ice Cold PBS Cell Scrapper Centrifuge High Salt Buffer Rotation Dilution Buffer Anti-flag Beads
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