Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Open Source High Content Analysis Utilizing…
JoVE Journal
Biology

Ce contenu est en libre accès.

JoVE Journal Biology
Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
 

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Article DOI: 10.3791/55119-v • 09:30 min • January 18th, 2017
January 18th, 2017 •
*1, *1, 1, 2, 3, 1, 1, 1, 1, 1, 1, 4, 4, 3, 5, 6, 1, 4, 1,7, 1
1Photonics Group, Department of Physics, Imperial College London, 2Institute for Chemical Biology, Department of Chemistry, Imperial College London, 3MRC Clinical Sciences Centre, Hammersmith Hospital, 4Chemical Biology Section, Department of Chemistry, Imperial College London, 5Retroscreen Virology Ltd, 6Pfizer Global Research and Development, Pfizer Limited, Sandwich, Kent, UK, 7Centre for Histopathology, Imperial College London
* These authors contributed equally

Chapitres

résumé
Automatic Translation

We present an open source high content analysis (HCA) instrument utilizing automated fluorescence lifetime imaging (FLIM) for assaying protein interactions using Förster resonance energy transfer (FRET) based readouts. Data acquisition for this openFLIM-HCA instrument is controlled by software written in µManager and data analysis is undertaken in FLIMfit.

Tags

Keywords: Open Source High Content Analysis Automated Fluorescence Lifetime Imaging Microscopy FLIM Multi-well Plate Global Analysis FRET Time-gated Live Cell Imaging Protein Interactions Cell Signaling Micromanager OpenFLIM-HCA Plugin Delay Box Calibration Instrument Response Function Spinning Disk Unit Fast Delay Box
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter