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JoVE Journal Cancer Research
In Vitro Imaging and Quantification of the Drug Targeting Efficiency of Fluorescently Labeled GnRH Analogues
 

In Vitro Imaging and Quantification of the Drug Targeting Efficiency of Fluorescently Labeled GnRH Analogues

Article DOI: 10.3791/55529-v • 10:36 min • March 21st, 2017
March 21st, 2017 •
1,2, 1, 1, 1, 3, 2
1MTA-SE Pathobiochemistry Research Group, Semmelweis University, 2Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, 3MTA-ELTE Research Group of Peptide Chemistry, Eötvös Loránd University

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résumé
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Selectively labeled fluorescent GnRH-I, -II and -III derivatives are reliable tools for tracking and quantifying their cellular uptake. This manuscript introduces experiments to visualize, quantify and compare the uptake efficiency of these GnRH conjugates in various cell lines.

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In Vitro Imaging Quantification Drug Targeting Efficiency Fluorescently Labeled GnRH Analogues Targeted Anti-cancer Therapy Confocal Microscopy Flow Cytometry Analysis GnRH Receptors Cancer Cell Types Selective Targeting Bianka Gurbi Ph.D. Student Cultured Medium Microscopic Slide Complete Medium GnRH Fit Seed Treating Media Incubator Carbon Dioxide Incubation Period PBS Solution
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