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JoVE Journal Genetics
Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
 

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Article DOI: 10.3791/55745-v • 12:02 min • June 6th, 2017
June 6th, 2017 •
*1, *2,3, 2
1Department of Cell Biology and Histology, University of the Basque Country, UPV/EHU, 2Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country, UPV/EHU, 3Department of Molecular Mechanisms of Disease, University of Zurich
* These authors contributed equally

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résumé
Automatic Translation

We report two cell synchronization protocols that provide a context for studying events related to specific phases of the cell cycle. We show that this approach is useful for analyzing the regulation of specific genes in an unperturbed cell cycle or upon exposure to agents affecting the cell cycle.

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Keywords: Cell Cycle Gene Expression Cell Synchronization U2OS Cells Thymidine Block Nocodazole Mitotic Cell Arrest G2-M RNA Extraction FACS Analysis
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