Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Visualizing Multiciliated Cells in the Zebrafish…
JoVE Journal
Developmental Biology

Ce contenu est en libre accès.

JoVE Journal Developmental Biology
Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
 

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

Article DOI: 10.3791/56261-v • 09:33 min • November 18th, 2017
November 18th, 2017 •
1, 1, 1, 1, 1
1Department of Biological Sciences, University of Notre Dame

Chapitres

résumé
Automatic Translation

Cilia development is vital to proper organogenesis. This protocol describes an optimized method to label and visualize ciliated cells of the zebrafish.

Tags

Keywords: Multiciliated Cells Zebrafish Whole Mount Fluorescent In Situ Hybridization Immunofluorescence Developmental Biology Pronephros Cilia RNA Transcripts Protein Labeling Antibodies Fixation Rehydration Proteinase K Hybridization Solution RNA Probe
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter