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JoVE Journal Cancer Research
A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s
 

A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s

Article DOI: 10.3791/56312-v • 07:38 min • September 25th, 2017
September 25th, 2017 •
1,2, 2, 2
1Department of Pharmacology, University of Minnesota, 2Masonic Cancer Center, University of Minnesota

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résumé
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α-hydroxylation of carcinogenic nitrosamines by cytochrome P450s is the accepted metabolic pathway that produces DNA-damaging intermediates, which cause mutations. However, new data indicates further oxidation to nitrosamides can occur. We describe a general method for detecting nitrosamides produced from in vitro cytochrome P450-catalyzed metabolism of nitrosamines.

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Keywords: Nitrosamide Nitrosamine Cytochrome P450 In Vitro Metabolism Mass Spectrometry Carcinogenesis Norcotinine Acetic Anhydride Sodium Nitrite Column Chromatography NMR Baculosomes NADPH Regeneration System
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