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Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
 

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Article DOI: 10.3791/56320-v • 10:42 min • December 31st, 2017
December 31st, 2017 •
1, 2, 1
1Division of Oral Surgery and Orthodontics, Department of Dental Medicine and Oral Health, Medical University of Graz, 2Division of Preventive and Operative Dentistry, Periodontology, Prosthodontics and Restorative Dentistry, Department of Dental Medicine and Oral Health, Medical University of Graz

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Changes in the oral microbiome throughout childhood are of growing interest. Comparison of different microbiome studies reveals a lack of standardized sampling protocols. Limited space makes sampling the sound subgingival sulcus of children challenging. Paper point sampling is presented here in detail as the method of choice for this area.

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Oral Biofilm Sampling Microbiome Analysis Healthy Children Standardized Method Instruments And Material Preparation Sterilization Biofilm Sampling Sites Subgingival Sulcus Mucosa From Vestibular Fold Saliva Systematic Storage Of Samples Oral Microbiome Analyses Orthodontist Orthodontic Treatment Limited Space In Subgingival Sulcus Lack Of Standardized Sampling Protocols Calibrated And Sterile Paper Points Endodontic Therapy UV Irradiation DNA And RNA Contamination Avoidance Teeth Cleaning Cocoa Butter Application Plaque Disclosure Staining
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