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Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking (TXL) for Myopia
 

Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking (TXL) for Myopia

Article DOI: 10.3791/56385-v • 12:25 min • January 6th, 2018
January 6th, 2018 •
1, 2, 1, 1
1Department of Ophthalmology, Columbia University College of Physicians and Surgeons, 2Confocal and Specialized Microscopy Shared Resource, Herbert Irving Comprehensive Cancer Center, Columbia University

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This protocol describes techniques for evaluating chemical cross-linking of the rabbit sclera using second harmonic generation imaging and differential scanning calorimetry.

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Keywords: Second Harmonic Generation SHG Microscopy Sclera Tissue Crosslinking TXL Myopia Collagen Sub-tenon's Injection Formaldehyde Differential Scanning Calorimetry Non-invasive Tissue Preparation Rabbit Eye Eyelid Speculum Conjunctiva Extra Ocular Muscles Scleral Insertion PBS Objective Lens Numerical Aperture Immersion Gel
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