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Live-Bilder von GLUT4 Protein Menschenhandel Maus primäre hypothalamische Neurone mit Dekonvolution Mikroskopie
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1The Ph.D. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology,Taipei Medical University, 2Integrated Laboratory, Center of Translational Medicine,Taipei Medical University, 3Core Facility,Taipei Medical University, 4The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, 5Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, 6TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology,Taipei Medical University
Chapitres
- 00:05Titre
- 01:00Primary Neuron Culture and Brain Tissue Isolation
- 02:56Tissue Digestion, Plating, and Culture
- 04:32Live Image Recording
- 06:48Results: The Snapshots of GFP-GLUT4 in Hypothalamic Neurons
- 07:47Conclusion
Dieses Protokoll beschreibt eine Technik für die Beobachtung von Echtzeit-grüne Fluoreszenz Protein (GFP) Glucose Transporter 4 (GLUT4) Protein Menschenhandel auf Insulin-Stimulation und Charakterisierung der biologischen Rolle der CCR5 in Insulin-GLUT4 markiert Signalweg mit Dekonvolution Mikroskopie.
