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Imagens ao vivo da proteína GLUT4 tráfico de Mouse de neurônios hipotálamo primário usando microscopia de deconvolução
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1The Ph.D. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology,Taipei Medical University, 2Integrated Laboratory, Center of Translational Medicine,Taipei Medical University, 3Core Facility,Taipei Medical University, 4The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, 5Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, 6TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology,Taipei Medical University
Chapitres
- 00:05Titre
- 01:00Primary Neuron Culture and Brain Tissue Isolation
- 02:56Tissue Digestion, Plating, and Culture
- 04:32Live Image Recording
- 06:48Results: The Snapshots of GFP-GLUT4 in Hypothalamic Neurons
- 07:47Conclusion
Este protocolo descreve uma técnica para observação de tempo real verde fluorescência proteína (GFP) com a tag 4 de transportador de glicose (GLUT4) proteína tráfico após estimulação da insulina e caracterização da função biológica de CCR5 na insulina – GLUT4 sinalização via com microscopia de deconvolução.
