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JoVE Journal Developmental Biology
Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications
 

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

Article DOI: 10.3791/56419-v • 08:41 min • December 4th, 2017
December 4th, 2017 •
1,2, 1,2, 2, 2, 1,2, 2,3,4,5, 2,3,4
1Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 2Berlin-Brandenburg Center for Regenerative Therapies (BCRT), 3German Center for Cardiovascular Research (DZHK), 4Deutsches Herzzentrum Berlin (DHZB), 5Department of Cardiovascular Surgery, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health

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This protocol describes the complete decellularization of human myocardium while preserving its extracellular matrix components. Further processing of the extracellular matrix results in the production of microparticles and a cytoprotective self-assembling hydrogel.

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Keywords: Cardiac Extracellular Matrix Hydrogel Cell-based Assays Myocardial Infarction Cryosectioning Tissue Processing Decellularization SDS Cardiac Tissue Engineering
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