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Neuroscience

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JoVE Journal Neuroscience
Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry
 

Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry

Article DOI: 10.3791/56538-v • 12:34 min • December 6th, 2017
December 6th, 2017 •
1, 1,2,3, 1,3
1Department of Medicine I and Clinical Chemistry, University Hospital of Heidelberg, 2Institute for Diabetes and Cancer IDC Helmholtz Center Munich, Germany & Joint Heidelberg-IDC Translational Diabetes Program, 3German Center for Diabetes Research (DZD)

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Automatic Translation

Quantitative analysis of cell content within the murine sciatic nerve is difficult due to the scarcity of the tissue. This protocol describes a method for tissue digestion and preparation that provides sufficient cells for flow cytometry analysis of immune cell populations from nerves of individual mice.

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Keywords: Flow Cytometry Immune Cells Sciatic Nerve Dorsal Root Ganglion Neuropathy Neuroinflammation Mouse Dissection Cell Isolation Digestion
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