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Neuroscience

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JoVE Journal Neuroscience
Use of Synaptic Zinc Histochemistry to Reveal Different Regions and Laminae in the Developing and Adult Brain
 

Use of Synaptic Zinc Histochemistry to Reveal Different Regions and Laminae in the Developing and Adult Brain

Article DOI: 10.3791/56547-v • 09:50 min • October 29th, 2017
October 29th, 2017 •
1, 2
1Department of Biology, Chemistry and Environmental Sciences, American University of Sharjah, 2Department of Biology, The City College of New York

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We describe a histochemical procedure that reveals characteristic laminar and areal zinc staining patterns in different brain regions. The zinc-staining pattern may be used in conjunction with other anatomical markers to reliably distinguish layers and regions in the developing and adult brain.

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Synaptic Zinc Histochemistry Brain Development Brain Regions Brain Layers Staining Patterns Ferret Frozen Brain Sections Qualitative Differences Quantitative Differences Demarcating Areas Visual Cortex Rodents Cats Primates Developer's Solution Gum Arabic Citric Acid Sodium Citrate Citrate Buffer Hydroquinone Silver Lactate
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