Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Cancer Research / Multiplexed Immunofluorescence Analysis and…
JoVE Journal
Cancer Research

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Cancer Research
Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells
 

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

Article DOI: 10.3791/56606-v • 09:32 min • February 8th, 2018
February 8th, 2018 •
1, 2,3,4, 2,3, 2,3, 2,3,5, 6, 7, 2, 2, 2, 2,3,6, 8, 2,3, 4, 2,6, *4, *2,4
1PARCC-INSERM U970, Université Paris Descartes, 2INSERM U970, Université Paris Descartes, 3Equipe Labellisée Ligue Contre le Cancer, 4Department of Immunology, Hopital Européen Georges Pompidou, 5Department of Medical Urology, Hopital Européen Georges Pompidou, 6Department of Pathology, Hopital Européen Georges Pompidou, 7Université Paris Diderot Paris 7, 8Department of medical oncology, Hopital Européen Georges Pompidou
* These authors contributed equally

Chapitres

résumé
Automatic Translation

Studying tumor microenvironment may identify prognostic or predictive biomarkers of clinical response to immunotherapy. Presented here, is an innovative method based on in situ fluorescence multispectral imaging to analyze and count automatically various subpopulations of CD8+ T cells. This reproducible and reliable technique is suitable for large cohort analyses.

Tags

Keywords: Multiplexed Immunofluorescence Cell Counting Tumor Microenvironment Phenotyping PD-1 Tim-3 CD8+ T Cells Immunological Interactions Tissue Sections Antibody Labeling Image Scanning
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter