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Cancer Research

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JoVE Journal Cancer Research
Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells
 

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells

Article DOI: 10.3791/58841-v • 09:57 min • January 29th, 2019
January 29th, 2019 •
*1,2, *3, 2, 3,4
1Royal (Dick) School of Veterinary Studies and Roslin Institute, University of Edinburgh, 2MRC Centre for Reproductive Health, University of Edinburgh, 3Edinburgh Phenotypic Assay Centre, University of Edinburgh, 4Cancer Research UK Edinburgh Centre, MRC Institute of Genetics, Molecular Medicine, University of Edinburgh
* These authors contributed equally

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résumé
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We describe here a protocol to investigate cytotoxicity of pre-activated CD8+ T cells against cancer cells by detecting apoptotic cancer cells via real-time microscopy. This protocol can investigate mechanisms behind myeloid cell-induced T cell suppression and evaluate compounds aimed at replenishing T cells via blockade of immune suppressive myeloid cells.

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Real-time Detection In Vitro Tumor Cell Apoptosis CD8+ T Cells Immune Suppressive Functions Tumor-infiltrating Myeloid Cells Myeloid-derived Suppressor Cells Tumor-associated Macrophages Therapeutic Interventions Longitudinal Analysis Cell-to-cell Interactions Checkpoint Inhibitor Efficacy Novel Therapies Cancer Immunology Research Immunodeficiency Autoimmune Diseases Activation And Expansion Of CD8-positive T-cells E-DMEM 96-well U-bottom Plate Suppression Cells
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