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DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering
 

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

Article DOI: 10.3791/56815-v • 10:35 min • November 9th, 2017
November 9th, 2017 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2
1Department of Chemistry and Biochemistry, Mendel University in Brno, 2Central European Institute of Technology, Brno University of Technology

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This protocol describes the synthesis of magnetic particles and evaluation of their DNA-binding properties via dynamic and electrophoretic light scattering. This method focuses on monitoring changes in particle size, their polydispersity and zeta potential of particle surface which play major role in binding of materials such as DNA.

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DNA-magnetic Particle Binding Analysis Dynamic Light Scattering Electrophoretic Light Scattering Biotechnology Nucleic Acid Magnetic Particles Physical-chemical Changes Size Polydispersity Zeta Potential Molecular Diagnosis Ferric Chloride Ferrous Chloride Deoxygenated Double Distilled Water Nitrogen Gas Atmosphere Mechanical Stirrer Aqueous Ammonia Screwable Vessel Isolation Of DNA
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