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Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila
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Sciences du comportement
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Journal JoVE Sciences du comportement
Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila

Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila

DOI:
10.3791/56900-v

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07:24 min

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April 24, 2018

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, ,
1School of Life Science,University of Science and Technology of China, 2State key Laboratory of Brain and Cognitive Science, Institute of Biophysics,Chinese Academy of Sciences, 3University of Chinese Academy of Sciences

Chapitres

  • 00:05Titre
  • 00:51Preparing the Feeding Chamber
  • 02:58Thermogenetic Activation
  • 03:44Optogenetic Activation
  • 04:48Colorimetric Quantification of Food Consumption
  • 06:08Results: Activated Taotie-GAL4 Neurons Increase Food Intake
  • 06:55Conclusion

Summary

Traduction automatique

Traduction automatique

Quantitative food-intake assays with dyed food provide a robust and high-throughput means to evaluate feeding motivation. Combining the food consumption assay with thermogenetic and optogenetic screens is a powerful approach to investigate the neural circuits underlying appetite in adult Drosophila melanogaster.

Tags

Food IntakeDrosophilaQuantitative AssayThermogenetic ActivationOptogenetic ActivationHungerSatietyAppetiteAgarose PadSucroseBlue DyeFeeding ChamberTemperature

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