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JoVE Journal Immunology and Infection
Generation of Monoclonal Antibodies Against Natural Products
 

Generation of Monoclonal Antibodies Against Natural Products

Article DOI: 10.3791/57116-v • 12:15 min • April 6th, 2019
April 6th, 2019 •
1, 2, 3, 4, 3, 3, 5
1School of Life Science, Beijing University of Chinese Medicine, 2Third Affiliated Hospital, Beijing University of Chinese Medicine, 3School of Basic Medical Sciences, Beijing University of Chinese Medicine, 4School of Chinese Materia Medica, Beijing University of Chinese Medicine, 5Center of Scientific Experiment, Beijing University of Chinese Medicine

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résumé
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This article provides a detailed protocol for the preparation and evaluation of monoclonal antibodies against natural products for use in various immunoassays. This procedure includes immunization, cell fusion, indirect competitive ELISA for positive clone screening, and monoclonal hybridoma preparation. The specifications for antibody characterization using MALDI-TOF-MS and ELISA analyses are also provided.

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Keywords: Monoclonal Antibodies ELISA Natural Products TCM Artificial Antigen Hapten Carrier Protein Mouse Immunization Cell Fusion Hybridoma Narigin BSA Conjugate Freund's Adjuvant
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