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Immunology and Infection
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JoVE Journal Immunology and Infection
Generation of Monoclonal Antibodies Against Natural Products
 

Generation of Monoclonal Antibodies Against Natural Products

Article DOI: 10.3791/57116-v • 12:15 min • April 6th, 2019
April 6th, 2019 •
1, 2, 3, 4, 3, 3, 5
1School of Life Science, Beijing University of Chinese Medicine, 2Third Affiliated Hospital, Beijing University of Chinese Medicine, 3School of Basic Medical Sciences, Beijing University of Chinese Medicine, 4School of Chinese Materia Medica, Beijing University of Chinese Medicine, 5Center of Scientific Experiment, Beijing University of Chinese Medicine

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résumé
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This article provides a detailed protocol for the preparation and evaluation of monoclonal antibodies against natural products for use in various immunoassays. This procedure includes immunization, cell fusion, indirect competitive ELISA for positive clone screening, and monoclonal hybridoma preparation. The specifications for antibody characterization using MALDI-TOF-MS and ELISA analyses are also provided.

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Monoclonal Antibodies Natural Products ELISA Qualitative Analysis Quantitative Analysis Foods Biological Samples Clinical Applications TCM Artificial Antigen Synthesis Mouse Immunization Cell Fusion Carrier Protein Hapten Adjuvants Spleen Single-cell Suspension Myeloma Cells HAT-sensitive Mouse Myeloma Cell Line ELISA Antibodies Hybridoma Cell Line Cryopreserved Narigin BSA Conjug
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