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Genetics

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JoVE Journal Genetics
En metod att studera den C924T polymorfism av tromboxan A2 receptorn gen
 
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En metod att studera den C924T polymorfism av tromboxan A2 receptorn gen

Article DOI: 10.3791/57289-v • 07:00 min • April 1st, 2019
April 1st, 2019 •
1, 1, 2, 2, 2, 1, 3, 1, 2, 4, 1,2
1SS Annunziata University Hospital, Unit of Clinical Molecular Biology and Predictive Medicine, University of Chieti, 2Department of Medical, Oral and Biotechnological Sciences, University of Chieti, 3Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, 4CNR - Institute of Molecular Genetics, Section of Chieti

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I denna studie, beskriver vi en metod för att analysera C924T genotypen. Protokollet består av tre faser: DNA-extraktion, förstärkning av polymeras-kedjereaktion (PCR) och analys begränsning fragment längd polymorfism (RFLP) på agarosgel.

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Genetik fråga 146 molekylärbiologi genuttryck restriktionsenzym analys Single Nucleotide Polymorphism genotypning trombocytaggregation Aterotrombos
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