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JoVE Journal Bioengineering
Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions
 

Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions

Article DOI: 10.3791/57314-v • 11:37 min • February 3rd, 2018
February 3rd, 2018 •
*1, *1, 1,2, 3,4, 1, 1, 1, 2, 2, 5, 6, 7,8
1Department of Chemical and Materials Engineering, National Central University, 2Department of Botany and Microbiology, King Saud University, 3Cathay Medical Research Institute, Cathay General Hospital, 4Graduate Institute of Systems Biology and Bioinformatics, National Central University, 5Department of Medical Microbiology and Parasitology, Universiti Putra Malaysia, 6Department of Internal Medicine, Taiwan Landseed Hospital, 7Department of Zoology, Bharathiar University, 8Thiruvalluvar University
* These authors contributed equally

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résumé
Automatic Translation

A protocol is presented to prepare polyvinyl alcohol-co-itaconic acid hydrogels with varying stiffness, which were grafted with and without oligopeptides, to investigate the effect of the stiffness of biomaterials on the differentiation and proliferation of stem cells. The stiffness of the hydrogels was controlled by the crosslinking time.

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Keywords: Polyvinyl Alcohol-co-itaconic Acid Hydrogels Stem Cell Culture Xeno-free Conditions Hydrogel Elasticity Stem Cell Differentiation Crosslinking Hydrogel Preparation P-IA Films
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