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Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining
 

Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining

Article DOI: 10.3791/57352-v • 07:46 min • March 5th, 2018
March 5th, 2018 •
1, 1,2,3, 1,2, 1,2
1Leonard Davis School of Gerontology, University of Southern California, 2Molecular & Computational Biology Section, University of Southern California, 3Integrative Program for Biological & Genome Sciences, University of North Carolina at Chapel Hill

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Nile red staining of fixed Caenorhabditis elegans is a method for quantitative measurement of neutral lipid deposits, while oil red O staining facilitates qualitative assessment of lipid distribution among tissues.

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Keywords: Lipid Abundance Lipid Distribution Caenorhabditis Elegans Nile Red Oil Red O Lipid Metabolism Lipid Homeostasis OP50 E.coli PBST Isopropanol NR Working Solution FITC GFP Channel
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