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Genetics

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JoVE Journal Genetics
CRISPR-Mediated Reorganization of Chromatin Loop Structure
 

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Article DOI: 10.3791/57457-v • 09:20 min • September 14th, 2018
September 14th, 2018 •
*1,2, *1, 1, 3, 4, 5, 1, 1, 1, 5, 3, 1,2,6
1Department of Dermatology, Program in Epithelial Biology, Stanford University School of Medicine, 2Program in Cancer Biology, Stanford University School of Medicine, 3Canary Center for Cancer Early Detection, Department of Radiology, Stanford University School of Medicine, 4Department of Biology, Bridgewater State University, 5System Biosciences, 6Veterans Affairs Healthcare System
* These authors contributed equally

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résumé
Automatic Translation

Chromatin looping plays a significant role in gene regulation; however, there have been no technological advances that allow for selective and reversible modification of chromatin loops. Here we describe a powerful system for chromatin loop re-organization using CRISPR-dCas9 (CLOuD9), demonstrated to selectively and reversibly modulate gene expression at targeted loci.

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Keywords: CRISPR Chromatin Loop Structure Gene Expression Transcriptional Dynamics Chromosomal Architecture Dimerizer Dcas9 Guide RNA Lentiviral CRISPR Plasmid Annealing Ligation
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