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Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
 

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Article DOI: 10.3791/57576-v • 13:47 min • June 3rd, 2018
June 3rd, 2018 •
*1, *2, *1, 2, 2, 2,3, 2,4, 1
1Immunity, Inflammation, and Disease Laboratory, Division of Intramural Research, National Institute of Environmental Health Sciences, NIH, 2Cellular and Molecular Pathology Branch, Division of the National Toxicology Program, National Institute of Environmental Health Sciences, NIH, 3Integrated Laboratory Systems Inc., 4Experimental Pathology Laboratories Inc.
* These authors contributed equally

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Here, we describe a protocol for a reproducible laser capture microdissection (LCM) for isolating trabecular meshwork (TM) for downstream RNA analysis. The ability to analyze changes in gene expression in the TM will help in understanding the underlying molecular mechanisms of TM-related ocular diseases.

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Keywords: Laser Microdissection Trabecular Meshwork Gene Expression Analysis Ocular Diseases RNA Isolation Cryostat Sectioning H&E Staining Laser Capture Microscopy
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