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Cancer Research

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JoVE Journal Cancer Research
Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors
 

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

Article DOI: 10.3791/57685-v • 08:32 min • June 7th, 2018
June 7th, 2018 •
*1,2, *1, 1
1Department of Otolaryngology-Head and Neck Surgery, Baylor College of Medicine, 2Interdepartmental Graduate Program in Translational Biology and Molecular Medicine, Baylor College of Medicine
* These authors contributed equally

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résumé
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Here we describe a method to separate and enrich components of the tumor immune and non-immune microenvironment in established subcutaneous tumors. This technique allows for the separate analysis of tumor immune infiltrate and non-immune tumor fractions which can permit comprehensive characterization of the tumor immune microenvironment.

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Keywords: Tumor Immune Microenvironment Tumor Non-immune Microenvironment Immune Oncology Tumor Profiling Tumor Dissociation Tumor Enrichment Tumor Cell Fractionation Tumor Immune Cell Separation Collagenase DNase Density Gradient Medium Subcutaneous Tumors
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