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JoVE Journal Neuroscience
Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish
 

Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish

Article DOI: 10.3791/57790-v • 07:14 min • August 16th, 2018
August 16th, 2018 •
*1, *1, 1
1Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Sciences
* These authors contributed equally

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The article describes an optimized protocol for making viable brain-pituitary tissue slices, using the teleost fish medaka (Oryzias latipes), followed by electrophysiological recordings of pituitary cells using the patch-clamp technique with the perforated patch configuration.

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Healthy Brain-pituitary Slices Electrophysiological Investigations Pituitary Cells Teleost Fish Neuroendocrinology Communication Between Pituitary Cells And Brain Releasing Factor Intact Environment Primary Cell Culture Electrical Properties Luteinizing Hormone Producing Cells Medaka Fish Fish Species Euthanized Teleost Fish Optic Nerves Dissecting Microscope Skull Bones Spinal Cord Ice Cold Extra Cellular Medium Agarose Mold
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