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Engineering

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JoVE Journal Engineering
A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms
 

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

Article DOI: 10.3791/57848-v • 06:50 min • February 11th, 2019
February 11th, 2019 •
*1, *1, *2, 1, 1
1Department of Biomedical Engineering and Institute for Complex Molecular Systems, Laboratory of Immunoengineering, Eindhoven University of Technology, 2Institute for Immunity, Transplantation, and Infection, Beckman Center, Stanford University
* These authors contributed equally

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résumé
Automatic Translation

This article presents a protocol for seeding scarce population of cells using pipette-tips to droplet microfluidic devices in order to provide higher encapsulation efficiency of cells in droplets.

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Pipette-tip Seeding Cells Droplet Microfluidic Platforms Systems Immunology Cellular Heterogeneity Communication Between Single Cells Pathogens Technique Development Rare Cells Encapsulation Tip Loading Procedure Visual Demonstration Aqueous Droplet Generation Surfactant Fluorinated Oil 2.5 Milliliter Syringe Teflon Tubing Bi-compatible Mineral Oil Polydimethylsiloxane (PDMS) Plug 200 Microliter Pipette-tip
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