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Developmental Biology

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JoVE Journal Developmental Biology
Visualization of Tangential Cell Migration in the Developing Chick Optic Tectum
 

Visualization of Tangential Cell Migration in the Developing Chick Optic Tectum

Article DOI: 10.3791/58506-v • 08:28 min • October 24th, 2018
October 24th, 2018 •
1, 1, 1
1Department of Neuroanatomy and Embryology, School of Medicine, Fukushima Medical University

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résumé
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We describe the methods for fluorescent labeling of tangentially migrating cells by electroporation, and for time-lapse imaging of the labeled cell movement in a flat-mount culture in order to visualize migrating cell behavior in the developing chick optic tectum.

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Keywords: Chick Optic Tectum Cell Migration Developmental Biology Alkaline Lysis Plasmid DNA Chicken Eggs Embryo Electroporation Optic Tectum Injection Micropipette PBS Forceps Electrodes Pulse Generator
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