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Immunology and Infection

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JoVE Journal Immunology and Infection
Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing
 

Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing

Article DOI: 10.3791/58569-v • 11:00 min • November 29th, 2018
November 29th, 2018 •
1, 1, 1, 1, 1, 1
1Institute for Molecular Medicine Finland (FIMM), HiLIFE, University of Helsinki

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We provide a method for the generation, cultivation and systematic analysis of organotypic slices derived from murine lung tumors. We also describe how to optimize for slice thickness, and how to select drug concentrations to treat tumor slices.

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Keywords: Tumor Slice Explants Preclinical Research Drug Response Studies Native Tissues Murine Lung Tumors Human Tissues Vibratome Hank's Balanced Salt Solution Euthanized Mouse Lung Heart Tissue Slicing Cyanoacrylate Adhesive Tumor Tissue
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