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Biochemistry

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JoVE Journal Biochemistry
Measuring and Interpreting Oxygen Consumption Rates in Whole Fly Head Segments
 

Measuring and Interpreting Oxygen Consumption Rates in Whole Fly Head Segments

Article DOI: 10.3791/58601-v • 07:11 min • January 7th, 2019
January 7th, 2019 •
1, 1, 2, 3,4,5, 1, 3, 2,6,7
1Munich Center of Integrated Protein Science and Biomedical Center, Ludwig-Maximilians University of Munich, 2Laboratory for Metabolism and Epigenetics in Aging, Leibniz Institute for Farm Animal Biology (FBN), 3German Mouse Clinic, Helmholtz Zentrum Munich, German Research Center for Environment and Health (GmbH), 4German Center for Diabetes Research (DZD), 5Chair of Experimental Genetics, School of Life Science Weihenstephan, Technische Universität München, 6Laboratory for Metabolism and Epigenetics in Brain Aging, Institute of Neuroregeneration & Neurorehabilitation of Qingdao University, 7Molecular Biology Division, Biomedical Center, Faculty of Medicine, Ludwig-Maximilians University of Munich

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Measuring alterations in metabolic rates is central to understanding the progression of various diseases and aging. Here, we present a novel technique to measure whole head oxygen consumption that more closely resembles the physiological state and may aid in revealing novel drugs that modify mitochondrial activity.

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Measurement Oxygen Consumption Rates Whole Fly Head Segments Metabolism Aging Intact Body Segment Mitochondrial Isolation Protocol Machine Temperature Software Setup Cartridge Calibration Injection Steps Basal OCR Drug Injection Calibrant Sensor Cartridge
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