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Cancer Research

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JoVE Journal Cancer Research
Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
 

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Article DOI: 10.3791/58615-v • 08:12 min • March 14th, 2019
March 14th, 2019 •
1, 1, 2, 3, 1
1Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 2Department of Medical Oncology, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 3Unit of Biostatistics and Clinical Trials, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS

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résumé
Automatic Translation

We present a protocol to evaluate the expression levels of circulating microRNA (miRNAs) in plasma samples from cancer patients. In particular, we used a commercially available kit for circulating miRNA extraction and reverse transcription. Finally, we analyzed a panel of 24 selected miRNAs using real-time pre-spotted probe custom plates.

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Circulating MicroRNA Custom Panel Metastatic Colorectal Cancer Translational Research Circulating Biomarkers Patient Prognosis Expression Evaluation Therapy Of Cancer Patients Decision-making Treatment Monitoring Insight Into Circulating Biomarkers Analysis Tissue Biomarker Expression Analysis Molecular Characterization Of Disease Visual Demonstration Extraction Steps Plasma Sample Denaturing Solution Spike-in Acid Phenol-chloroform Vortexing Centrifugation Ethanol
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