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Bioengineering

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JoVE Journal Bioengineering
High-resolution Patterned Biofilm Deposition Using pDawn-Ag43
 

High-resolution Patterned Biofilm Deposition Using pDawn-Ag43

Article DOI: 10.3791/58625-v • 07:48 min • October 23rd, 2018
October 23rd, 2018 •
1, 1
1Department of Bioengineering, Stanford University

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résumé
Automatic Translation

We demonstrate a method for depositing Escherichia coli bacterial biofilms in arbitrary spatial patterns with a high resolution using optical stimulation of a genetically encoded surface-adhesion construct.

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Biofilm Lithography High-resolution Patterned Biofilm Deposition PDawn-Ag43 Spatial Structures In Biofilm Research Advantages Of Biofilm Lithography Surface Pre-patterning Closed Fluidic Devices Insight Into Structure-function Relationship Biofilm Engineering Biomaterials Multicellular Synthetic Biology Optical Culture Setup Bacterial Sample Preparation PDawn-Ag43 Plasmid Transformation LB Agar Plate Spectinomycin Supplementation Exponential Phase Growth Shaking Incubator
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