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Immunology and Infection

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JoVE Journal Immunology and Infection
Listeria monocytogenes Infection of the Brain
 

Listeria monocytogenes Infection of the Brain

Article DOI: 10.3791/58723-v • 05:02 min • October 2nd, 2018
October 2nd, 2018 •
1, 1
1Department of Microbiology and Immunobiology, Harvard Medical School

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résumé
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During infection, Listeria monocytogenes is capable of crossing the blood-brain barrier to colonize the brain. In this protocol, we demonstrate how to assess bacterial colonization of organs following infection of mice. A procedure to perform whole organ perfusion for specific determination of bacterial numbers in the brain parenchyma is provided.

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Listeria Monocytogenes Bacterial Pathogenesis Blood-brain Barrier Central Nervous System Brain Infection Automated Perfusion System PBS EDTA Euthanization Abdominal Wall Incision Diaphragm Thoracic Cavity Left Ventricle Right Atrium Blood Collection Perfusion
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