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Neuroscience

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JoVE Journal Neuroscience
An Unbiased Approach of Sampling TEM Sections in Neuroscience
 

An Unbiased Approach of Sampling TEM Sections in Neuroscience

Article DOI: 10.3791/58745-v • 10:56 min • April 13th, 2019
April 13th, 2019 •
1, 2, 1, 3, 4,5, 4, 4, 4, 4, 2, 1, 1, 1
1Department of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center, Medical University of Graz, 2Department of Pharmacology, Otto Loewi Research Center, Medical University of Graz, 3Division of General Neurology, Department of Neurology, Medical University of Graz, 4Institute of Pathology, Medical University of Graz, 5Department of Pathology, Medical Faculty, Otto von Guericke University Magdeburg

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résumé
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We introduce a novel workflow for electron microscopy investigations of brain tissue. The method allows the user to examine neuronal features in an unbiased fashion. For elemental analysis, we also present a script that automatizes most of the workflow for randomized sampling.

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Keywords: - Unbiased Sampling - Electron Microscopy - Neuroscience - Quantification - Numerical Density - Synapses - Neural Network - Sample Preparation - Ultramicrotome - Serial Sections - Slot Grid - Counterstaining - Transmission Electron Microscope (TEM) - TEM Serial Section Software - Region Of Interest (ROI) - Random Point Sampling
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