JoVE Journal
Bioengineering
Bioengineering
Ce contenu est en libre accès.
Chapitres
résumé
A detailed protocol for the purification and subsequent analysis of a monoclonal antibody from harvested cell culture fluid (HCCF) of automated microbioreactors has been described. Use of analytics to determine critical quality attributes (CQAs) and maximizing limited sample volume to extract vital information is also presented.