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JoVE Journal Genetics
A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
 

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Article DOI: 10.3791/59055-v • 07:10 min • February 19th, 2019
February 19th, 2019 •
1, 1, 1, 1
1Department of Biology, Georgetown University

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Described here is a method for analyzing bacterial gene expression in animal tissues at a cellular level. This method provides a resource for studying the phenotypic diversity occurring within a bacterial population in response to the tissue environment during an infection.

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Keywords: Bacterial Gene Regulation Fluorescence-based Method Bacterial Gene Expression Tissue Microenvironments S. Aureus Hemolytic Phenotype Bacterial Physiology Antivirulence Antibacterial Compounds Tissue Fixation Cryosectioning DAPI Staining
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