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Developmental Biology

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JoVE Journal Developmental Biology
Wholemount i Situ hybridisering til Astyanax embryoner
 
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Wholemount i Situ hybridisering til Astyanax embryoner

Article DOI: 10.3791/59114-v • 09:56 min • March 2nd, 2019
March 2nd, 2019 •
1, 1, 1, 1
1Department of Biological Sciences, University of Cincinnati

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Denne protokol giver mulighed for visualisering af genekspression i embryonale Astyanax cavefish. Denne fremgangsmåde er blevet udviklet med formålet at maksimere gen expression signal, samtidig minimere uspecifikke baggrund farvning.

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Udviklingsmæssige biologi sag 145 Gen-ekspression kromatisk farvning WMISH cavefish mRNA sonde morfologi
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